PowerFit - Results 3zpz_C

GroEL/ES chaperonin system

In this example chain C of the GroEL/ES chaperonin system (PDB entry 3zpz) was fitted into the corresponding cryo-EM density map of the full complex (EMDB entry 2325 - 8.9 Å resolution) with a rotational sampling interval of 5 degrees. While Powerfit clearly favors one location based on cross correlation score and sigma difference, there is still a major break in sigma difference between the 7 symetric orientations and the next best fit (Fit 8).

Run 3zpz_C - EMD-2325 (Example3)

Status: FINISHED

Your PowerFit run has successfully completed.

Archive of the complete run:	Example3.tgz
Archive of all autogenerated images:	Example3_images.tgz

Please cite the following papers in your work:

G.C.P. van Zundert, M. Trellet, J. Schaarschmidt, Z. Kurkcuoglu, M. David, M. Verlato, A. Rosato and A.M.J.J. Bonvin.
The DisVis and PowerFit web servers: Explorative and Integrative Modeling of Biomolecular Complexes. J. Mol. Biol., Advanced Online Publication (2016).

G.C.P. van Zundert and A.M.J.J. Bonvin (2015)
Fast and sensitive rigid-body fitting into cryo-EM density maps with PowerFit.
AIMS Biophysics 2, 73-87.

and add the following acknowledgment:

The FP7 WeNMR (project# 261572), H2020 West-Life (project# 675858), EOSC-hub (project# 777536) and the EGI-ACE (project# 101017567) European e-Infrastructure projects are acknowledged for the use of their web portals, which make use of the EGI infrastructure with the dedicated support of CESNET-MCC, INFN-LNL-2, NCG-INGRID-PT, TW-NCHC, CESGA, IFCA-LCG2, UA-BITP, TR-FC1-ULAKBIM, CSTCLOUD-EGI, IN2P3-CPPM, CIRMMP, SURFsara and NIKHEF, and the additional support of the national GRID Initiatives of Belgium, France, Italy, Germany, the Netherlands, Poland, Portugal, Spain, UK, Taiwan and the US Open Science Grid.

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Solutions

The table below lists the 15 best non-redundant solutions found by correlation score. The first column shows the rank, column 2 the correlation score, column 3 the Fisher z-score column 4 the zscore as factor of standard deviations (z/σ), and column 5 the sigma difference to the best fit. (see N. Volkmann 2009, and Van Zundert and Bonvin 2016).

Rank (N)	Cross Correlation Score	Fisher z-score	z-score/σ	Sigma difference (z₁-z_N)/σ
1	0.507	0.559	38.4	0.00
2	0.489	0.535	36.8	1.65
3	0.478	0.520	35.7	2.69
4	0.477	0.519	35.7	2.74
5	0.466	0.505	34.7	3.75
6	0.466	0.505	34.7	3.75
7	0.457	0.493	33.9	4.52
8	0.326	0.338	23.2	15.17
9	0.324	0.336	23.1	15.34
10	0.315	0.326	22.4	15.99
11	0.314	0.325	22.3	16.07
12	0.313	0.324	22.2	16.17
13	0.312	0.323	22.2	16.21
14	0.311	0.322	22.1	16.29
15	0.307	0.317	21.8	16.65